Clean Genome® E. coli
Ran, H. et al. Enhanced Production of Recombinant Thermobifida fusca Isoamylase in Escherichia coli MDS42. Appl Biochem Biotechnol 180, 464–476 (2016).
Ying et al. (2013) Multilevel Comparative Analysis of the Contributions of Genome Reduction and Heat Shock to the E. coli Transcriptome. BMC Genomics, 14:25.
Lee, J.H. et al. Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production. Microb Cell Fact 8, 2 (2009).
Pósfai G, et al., (2006) Emergent Properties of Reduced-Genome Escherichia coli. Science 312:1044-6.
Kolisnychenko, V., et al., 2002. Engineering a reduced Escherichia coli genome. Genome Res. 12(4): 640-7.
Blattner, F.R., et al., 1997. The complete sequence of Escherichia coli K-12. Science 277 (5331): 1453-1474.
Protein and Nucleic Acids Production
Chacko S. Chakiath, CS & Esposito, D (2007): Improved Recombinational Stability of Lentiviral Expression Vectors Using Reduced-Genome Escherichia coli. BioTechniques 43:466-470.
Sharma, S., et al., 2006. Recombinant protein production in an Escherichia coli reduced genome strain. Metabolic Engineering 9 (2007) 133-141
Reduced Mutant Frequency
Csörgo et al. (2012) Low-Mutation-Rate, Reduced-Genome Escherichia coli an Improved Host for Faithful Maintenance of Engineered Genetic Constructs. Microbial Cell Factories, 11:11.
IS Elements
van der Heijden et al. Transposon Leads to Contamination of Clinical pDNA Vaccine. (2013) Vaccine 31:3274-80.
Umenhoffer et al. (2010) Reduced Evolvability of Escherichia coli MDS42, an IS-Less Cellular Chassis for Molecular and Synthetic Biology Applications. Microbial Cell Factories, 9:38.
Prather, K.L.J. et al., (2006) Identification and characterization of IS1 transposition in plasmid amplification mutants of E. coli clones producing DNA vaccines. Appl Microbiol Biotechnol. 73(4):815-26.