Name: Clean Genome<sup>®</sup> LowMut T7
SKU: 10122
Availability: InStock

Clean Genome^® LowMut T7

$890.00 – $3,350.00

Clean Genome^® LowMut T7 provides a T7 promoter in the genome for clients wishing to use this expression option rather than the recommended synthetic T5 promoter in the pSX2 vector. Like Clean Genome^® LowMut, this strain reduces the already low mutation rate of Clean Genome^® strains. It has been engineered to reduce error-prone repair, thereby reducing the mutation rate to almost zero, even under the most stressful conditions. Note that product yields may be lower with T7 promoters compared with the same gene cloned into pSX2.

Description

Kit Components

Chemically Competent Cells	C-10122-05	C-10122-10	C-10122-20
Electrocompetent Cells	E-10122-05	E-10122-10	E-10122-20
Cells	1 x 0.25 mL	2 x 0.25 mL	4 x 0.25 mL
pUC19 Control DNA (10 pg/µL)	1 x 50 µL	1 x 50 µL	1 x 50 µL
SOC Medium	1 x 10 mL	1 x 10 mL	2 x 10 mL

Quality Control

Transformation efficiency is tested using pUC19 control DNA, performed in duplicate. Transformed cells are plated on LB agar containing 50 μg/ml carbenicillin. Transformation efficiency is ≥ 1 x 10⁸ cfu/μg DNA.

Storage Conditions

Store components at -80 °C. Do not store cells in liquid nitrogen.

Download product protocols

• Chemically Competent Cells Product Protocol
• Electrocompetent Cells Product Protocol

Ordering

A Limited License Agreement is required for research, and a commercial license must be negotiated for commercial use.

Note: The last two numbers in each catalog number indicate the number of transformations the kit will support.

Additional information

Competent Cell Type	Chemically Competent Cells, Electrocompetent Cells
No. of transformations	5, 10, 20